Application

Research CategoryInflammation & Immunology

Produced in collaboration with Epitomics™

Anti-TFPI, clone EPR7941, Rabbit Monoclonal Antibody is an antibody against TFPI for use in Western Blotting.

Research Sub CategoryInflammation & Autoimmune Mechanisms

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tissue factor pathway inhibitor (TFPI), also known as Extrinsic pathway inhibitor or EPI or Lipoprotein-associated coagulation inhibitor or LACI, and encoded by the gene name TFPI or LACI or TFPI1, is a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. TFPI inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. It is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins.

Immunogen

Epitope: C-terminus

Synthetic peptide corresponding to the C-terminus of human TFPI.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.

Format: Unpurified

Unpurified

Quality

Evaluated by Western Blotting in HepG2, U-87 MG, Caco-2, and human placenta lysates.

Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected TFPI in HepG2, U-87 MG, Caco-2, and human placenta lysates.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

~40 kDa observed. Uncharacterized bands may be observed in some lysate(s).